Get Full Essay Get access to this section to get all help you need with your essay and educational issues. The juvenile stage has proved to the be the best time to take cuttings for propagation. Manipulation of stock plant juvenility and light has proved to be a powerful tool in successful propagation on the otherwise difficult to root woody species. Young and fleshy cuttings as opposed to stalky cuttings from older plants root successfully.
Determine how gene expression in cuttings changes as donor trees age. Project Methods Genes expressed during adventitious root initiation in pine cuttings will be identified by cDNA cloning and by using gene probes from other plants.
The temporal and spatial expression of root initiation genes will be studied in easily rooted cuttings from juvenile trees and recalcitrant cuttings from mature trees. The molecular obstacles to rooting in cuttings from mature trees will be determined by elucidating the regulatory mechanisms of the root initiation gene expression pathway.
Outputs for this project have been disseminated in three ways. First, practical knowledge about propagation techniques and clone longevity have been transferred to industrial and agency cooperators. Second, although cost of production factors have prohibited the large-scale implementation of the knowledge in the U.
Third, due to lower costs of production in South America, the knowledge is being used on a commercial scale in some countries.
Fourth, the more basic findings on root development have been disseminated to the broader scientific community. In all cases, the most significant findings have been communicated via workshops, presentations at scientific conferences and journal articles.
This project involved several university scientist faculty members and scientists from companies and agencies. Technology transfer was accomplished by on-site visits to collaborators and by specific annual meetings.
A key goal was professional training of undergraduate and graduate students. They gained research expertise, experience in interacting with stakeholders, and, in some case, were hired by collaborating organizations.
Supporting companies, both inside and outside of the U. Nothing significant to report during this reporting period. Impacts a Optimal nutritional treatments were determined for loblolly pine stock plants. This has led to specific grower recommendations for the best rooting success.
This has led to customizable growing parameters that growers can adapt to their specific propagation environment. Rooting experiments with clones of different ages showed a slight decline in rooting between two- and three-year-old clones.
However, no further decline was observed through ten years of age. A field test of rooted cuttings from clones through eleven years of age showed the same trend.
This finding has resulted in establishment of boundaries for propagation of clones by rooted stem cuttings. These are excellent candidates for further study.
In addition, initial transgenic experiments with three root development genes has generated plants with altered primary to lateral root ratios. Further characterization of these phenotypes will occur under future projects.
Predicted genetic gains and testing efficiency from two loblolly pine clonal trials. Genetic effects of rooting of loblolly pine stem cuttings from a partial diallel mating design.
Analysis of cellulose microfibril angle using a linear mixed model in Pinus taeda clones. Prediction of wood density breeding values of Pinus taeda elite parents from unbalanced data: A method for adjustment of site and age effects using common checklots.
Genetic analysis of early field growth of loblolly pine clones and seedlings from the same full-sib families. Genetic gain from selection for rooting ability and early growth in vegetatively propagated clones of loblolly pine.
Tree Genetics and Genomes 3: Enhancer trapping in woody plants: Isolation of the ET gene encoding a putative AT-hook motif transcription factor and characterization of the expression patterns conferred by its promoter in transgenic Populus and Arabidopsis.
Container type and volume influences adventitious rooting and subsequent field growth of stem cuttings of loblolly pine. Improving the rooting capacity of Virginia pine by severe stumping of parent trees.
Morphological and chemical variations between juvenile wood, mature wood, and compression wood of loblolly pine Pinus taeda L. Utilization of polar metabolite profiling in the comparison of juvenile wood and compression wood in loblolly pine Pinus taeda.
Rapid prediction of wood properties of loblolly pine using transmittance near infrared spectroscopy. Research on clone age showed a singificant but slower than anticipated decline in rooting ability with increasing clone age.-management of stock plants - shoots to be used for cuttings are girdled @ base several weeks before cuttings are taken - rooting is enhanced by blocking downward movement of carbs, hormones, rooting .
Data on stock plants from the clonal garden and mini-clonal garden were correlated with data on rooting parameters of cuttings and mini-cuttings to determine which factors of the stock plant and the shoot may have influenced rooting.
Juvenility affects the rooting ability of cuttings the way that young plants produce cuttings that are easier to root than the ones from old stock plants (Hess , Hess , Schier , Tervonen ).
In woody plants, the juvenile traits, including rooting capability, are expressed at the base of the stem throughout the life of the plant and are sharply or gradually replaced by maturity traits, including rooting inability, toward the upper part of the main stem and branches.
The environment which supports and facilitates rooting of cuttings has a profound influence on the success or failure of the establishment of a new plant.
The purpose of this chapter is to assess the types of variation in environment that are important. The intention is to focus on the entire.
Helpful Suggestions for Commercial Propagation of Woody Plant Stem Cuttings 2 water, and nutrients). Use blemish-free cuttings taken from non-stressed plants that exhibit uniform growth.